Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

Scientific Article | Ce protocole décrit en détail la préparation des complexes nucléosomiques à l’aide de deux méthodes de préparation des échantillons
DNA repair in the context of chromatin is poorly understood. Biochemical studies using nucleosome core particles, the fundamental repeating unit of chromatin, show most DNA repair enzymes remove DNA damage at reduced rates as compared to free DNA.

Structural and mechanistic insights into the DNA glycosylase AAG

Cryo-EM structure of SWI/SNF complex bound to a nucleosome

The integrative role of cryo electron microscopy in molecular and

Preparation of Nucleosome Core Particles Complexed with DNA Repair

The nucleosome-bound structures for the various epigenetic factors

Cryo-EM analysis of nucleosomal particles. a,b. Three

Model for modulation of Polymerase enzymatic activities by the

Structure of native chromatin fibres revealed by Cryo-ET in situ

The cryo-EM resolution revolution and transcription complexes

Structural and mechanistic insights into the DNA glycosylase AAG

Frontiers Nucleosomes and their complexes in the cryoEM era

Frontiers Recent Technical Advances in Sample Preparation for

Preparation of Nucleosome Core Particles Complexed with DNA Repair

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